Data represent three independent experiments (*Different expression of epithelial and mesenchymal markers. (A) Expression of genes relating to cell adhesion in non-SP cells compared with those in SP cells was performed by microarray analysis from HO-8910PM cell line. The role of adherent junction proteins has been studied extensively in cancer, but the roles of tight junction (TJ) proteins are less well understood. Page d’accueil Équipes Polarité Cellulaire, Signalisation et Cancer Plus de 90% des tumeurs humaines chez l’adulte ont pour origine la transformation maligne de cellules épithéliales par la dérégulation de gènes appelés oncogènes et gènes suppresseurs de tumeur. Here, we report an increased expression of claudin-1 in human primary colon carcinoma and metastasis and in cell lines derived from primary and metastatic tumors.
eCollection 2020.Bagheri M, Fazli M, Saeednia S, Gholami Kharanagh M, Ahmadiankia N.Bukosza EN, Kratochwill K, Kornauth C, Schachner H, Aufricht C, Gebeshuber CA.PLoS One.

These findings illustrate the interplay between epithelial ovarian CSCs and the EMT, and exert a link to explain tumor heterogeneity and its necessity for ovarian cancer maintenance, metastasis and progression.

Cells migrated into membrane were stained with Hematoxylin and counted under microscope. doi: 10.1371/journal.pone.0231898. (Effect of inhibition of claudin-1 on E-cadherin expression and β-catenin/Tcf/Lef activity. Claudin-1 regulates cellular transformation and metastatic behavior in colon cancer.

We have identified that ovarian cancer stemlike cells (CSCs), which were defined as side population (SP) cells, were present in patients' ascitic fluid and mesenchymally transformed cell lines, ES-2 and HO-8910PM. 2018 Oct;33(10):1013-1019. doi: 10.14670/HH-11-980. 2019 Jul 26;11(7):383-397. doi: 10.4252/wjsc.v11.i7.383.Schiffman C, Lin C, Shi F, Chen L, Sohn L, Huang H.Stat Biosci. Furthermore, we demonstrate that changes in claudin-1 expression have significant effects on growth of xenografted tumors and metastasis in athymic mice. DOI: 10.1172/JCI24543 Corpus ID: 7368199. SP cells, which were sorted from both cell lines and implanted into immunocompromised mice, were localized to the xenografted tumor boundary.

On sait aujourd’hui que la transformation des cel-lules est liée à la présence de gènes cancer ou … 2020 Apr 23;13:3437-3448. doi: 10.2147/OTT.S242406. Claudins are recently identified members of the tetraspanin family of proteins, which are integral to the structure and function of TJs. Epub 2014 Oct 21.BMC Cancer.

La transformation cellulaire désigne ici les étapes successives de la cellule différenciée saine jusqu'au stade cancéreux.. Contrairement aux maladies génétiques comme la mucoviscidose, les myopathies ou certaines hémophilies qui sont des maladies monogéniques (un seul gène est généralement altéré), le cancer est une maladie multigénique. (Effects of siRNA-based inhibition of claudin-1 expression in SW620 cells on proliferation, anchorage-independent growth, and invasion. Recent studies show changes in expression/cellular localization of claudins during tumorigenesis; however, a causal relationship between claudin expression/localization and cancer has not been established.

2011 Sep 19;11:396. doi: 10.1186/1471-2407-11-396.Hum Pathol. La Transformation cellulaire Le cancer est lié à la prolifération anarchique et incontrôlée des cellules résultant d’uneperturbationdel’homéostasietissulaire L’Homéostasietissulaire est Un Fragile équilibre entre: La prolifération cellulaire La différenciation ou la spécialisation irréversible des cellules Malignant cancer cells arise from normal cells via a multistep process that involves both genetic and epigenetic change. We also report frequent nuclear localization of claudin-1 in these samples. (A) Expression of genes encoding Snail1,… Gene expression profiles during TGF-β1 stimulation. 2020 May 23;12(5):1339. doi: 10.3390/cancers12051339.Onco Targets Ther. 2019 Nov;21(11):1073-1084. doi: 10.1016/j.neo.2019.10.001. SP component presented in patients’ ascitic fluid and localized on tumor boundary.

(Effects of siRNA-based inhibition of claudin-1 expression in the metastatic SW620 cells.

The role of adherent junction proteins has been studied extensively in cancer, but the roles of tight junction (TJ) proteins are less well understood.
(A) Expression of genes encoding Snail1, Snail2, S100A4, SIP1, SMAD2, SMAD3, KRT8 and KRT15 in HO-8910PM-SP cells relative to non-SP cells were determined by real-time RT-PCR (Gene expression profiles during TGF-β1 stimulation. Scale bar = 50 μm. 2019 Nov 12;11(11):1781. doi: 10.3390/cancers11111781.World J Stem Cells. SP component presented in patients’ ascitic fluid and localized on tumor boundary.

(B) Microarray analyses were performed to compare gene expression profiles of freshly sorted SP cells, freshly sorted non-SP cells, TGF-β1 induced SP cells (all from HO-8910PM cells) and human fibroblast cell line, HS-27. Disruption of the cell-cell junction with concomitant changes in the expression of junctional proteins is a hallmark of cancer cell invasion and metastasis. This site needs JavaScript to work properly. Each column of ascetic fluid and primary tumor was obtained from the same patient. This heterogeneity was observed as an endogenous transformation via the epithelial-mesenchymal transition (EMT) process. (